Publications

Selected publications from the laboratory:

Iadecola C., Smith EE., Anrather J., Gu C, Mishra A., Misra S., Perez-Pinzon MA., Shih AY., Sorond FA., van Veluw SJ., Wellington CL.; American Heart Association Stroke Council on Arteriosclerosis Thrombosis and Vascular Biology Council on Cardiovascular Radiology and Intervention Council on Hypertension Council on Lifestyle and Cardiometabolic Health. The Neurovasculome: Key roles in brain health and cognitive Impairment: A scientific statement from the American Heart Association/American Stroke Association. Stroke, 54(6):251-271. (2023). (Download PDF)


Krolak T., Chan KY., Kaplan L., Huang Q., Wu J., Zheng Q., Kozareva V., Beddow T., Tobey IG., Pacouret S., Chen AT., Chan YA., Ryvkin D., Gu C., Deverman BE (2022). A high-efficiency AAV for endothelial cell transduction throughout the central nervous system. Nature Cardiovascular Research. 1, 389–400 (2022). (Download PDF).
 

Ayloo, S., Gallego Lazo C., Sun, S., Zhang, W., Cui, B., Gu, C., (2022) Pericyte-to-endothelial cell signaling via vitronectin-integrin regulates blood-CNS barrier. Neuron. 110: 1–15 (Download PDF).


Wood C.A.P, Zhang J., Aydin D., Xu Y., Andreone B.J, Langen U.H, Dror R.O, Gu C., Feng L. (2021) Structure and mechanism of blood-brain-barrier lipid transporter MFSD2A. Nature. 596(7872):444-448 (Download PDF)


Mäe M.A, He L., Nordling S., Vazquez-Liebanas E., Nahar K., Jung B., Li X., Tan BC., Chin Foo J., Cazenave-Gassiot A., Wenk M.R., Zarb Y., Lavina B., Quaggin S.E., Jeansson M., Gu C., Silver D.L., Vanlandewijck M., Butcher E.C., Keller A., Betsholtz C. (2021). Single-cell analysis of blood-brain barrier response to pericyte loss. Circ Res. 128(4):e46-e62. (Download PDF)


Kaplan, L., Chow B.W., Gu C. (2020). Neuronal regulation of the blood-brain barrier and neurovascular coupling. Nature Reviews Neuroscience. 21(8):416-432. (Download PDF)
 

Chow, B.W., Nuñez, V., Kaplan, L., Granger, A.J., Bistrong, K., Zucker, H.L., Kumar, P., Sabatini, B.L., Gu C. (2020) Caveolae in CNS arterioles mediate neurovascular coupling. Nature, 579(7797):106-110. (Download PDF)
 

Granger A.J, Wang W., Robertson K., El-Rifai M., Zanello A.F., Bistrong K., Saunders A., Chow B.W., Nuñez V., Turrero García M., Harwell C.C., Gu C., Sabatini B.L (2020). Cortical ChAT + neurons co-transmit acetylcholine and GABA in a target- and brain-region-specific manner. eLife. 9:e57749. (Download PDF)
 

O'Brown N. M., Megason S.G., Gu C. (2019) Suppression of transcytosis regulates zebrafish blood-brain barrier function. eLife, 8:e47326. (Download PDF)
 

Langen U., Ayloo S., Gu C., (2019) Development and cell Biology of the blood-brain barrier. Annual Review of Cell and Developmental Biology, 35:591-613. (Download PDF)
 

Ayloo, Swathi, Gu C., (2019) Transcytosis at the blood–brain barrier. Current Opinion in Neurobiology, 35:591-613.  (Download PDF)
 

Sadeghian H, Lacoste B, Qin T, Toussay X, Rosa R, Oka F, Chung DY, Takizawa T, Gu C, Ayata C., (2018) Spreading depolarizations trigger caveolin-1–dependent endothelial transcytosis. Annals of Neurology, 84(3):409-423. (Download PDF)

O'Brown, Natasha M., Pfau, Sarah J., Gu C., (2018) Bridging barriers: a comparative look at the blood–brain barrier across organisms. Genes & Development, 32(7-8):466-478. (Download PDF)
 

Andreone, B.J., Chow, B.W., Tata, A., Lacoste, B., Ben-Zvi, A., Bullock, K., Deik, A.A., Ginty, D.D., Clish, C.B., Gu C., (2017) Blood-brain barrier permeability is regulated by lipid transport-dependent suppression of caveolae-mediated transcytosis. Neuron, 94(3) 581-594. (Download PDF)

News report on this study: "Unlocking the Barrier"
 

Chow,B.C, Gu C., (2017) Gradual suppression of transcytosis governs functional blood-retinal barrier formation. Neuron, 93(6)1325-1333. (Download PDF)

News report on this study: "Filtering more than light in the developing retina"-commentary in Science Translational Medicine.
 

Kur, E., Kim, J., Tata, A., Comin, C.H., Harrington, K.I., Costa, L.,, Bentley. K., Gu C., (2016) Temporal modulation of collective cell behavior controls vascular network topology. Elife. 5:e13212. (Download PDF)
 

Chow.B.W., Gu, C., (2015) The molecular constituents of the blood-brain barrier. Trends Neurosci. 38(10):598-608. (Download PDF)
 

Lacoste, B., Gu, C., (2015) Control of cerebrovascular patterning by neural activity during postnatal development. Mech Dev. 138 Pt 1:43-9. (Download PDF)
 

Andreone, B.J., Lacoste, B., Gu, C., (2015) Neuronal and vascular interactions. Annu Rev Neurosci.38:25-46. (Download PDF)
 

Keskin, D., Kim, J., Cooke, V.G., Wu, C.C., Sugimoto, H., Gu C, De Palma, M., Kalluri, R., LeBleu, V.S., (2015) Targeting vascular pericyte in hypoxic tumors increases lung metastasis via angiopoietin-2. Cell Rep. 10(7):1066-81. (Download PDF)
 

Almasi S., Xu X., Ben-Zvi A., Lacoste B., Gu C., Miller E.L., (2015) A novel method for identifying a graph-based representation of 3-D microvascular networks from fluorescence microscopy image stacks. Med Image Anal, 20(1):208-23. (Download PDF)
 

Gelfand, M.V., Hagan, N., Tata, A., Oh, W.J., Lacoste, B., Kang, K.T., Kopycinska, J., Bischoff, J., Wang, J.H., Gu C., (2014) Neuropilin-1 functions as a VEGFR2 co-receptor to guide developmental angiogenesis independent of ligand binding. Elife. 3:e03720. (Download PDF)

Lacoste, B., Comin, C.H., Ben-Zvi, A., Kaeser, P.S., Xu, X., Costa, L., and Gu, C., (2014) Sensory-related neural activity regulates the structure of vascular networks in the cerebral cortex. Neuron, 83(5):1117-30. (Download PDF)
News report on this study.
Video abstract
Science News on this study
 

Ben-Zvi, A., Lacoste, B., Kur, E., Andreone, B.J., Mayshar, Y., Yan, H., Gu, C., (2014) MFSD2A is critical for the formation and function of the blood brain barrierNature, 509(7501):507-11. (Download PDF)
News & Views in Nature 509(7501):432-3.
Preview in Neuron 82(4):728-30.
News report on this study.
 

Tata, A., Stoppel, D., Hong, S., Ben-Zvi, A., Xie, T., Gu, C., (2014) An image-based RNAi screen identifies SH3BP1 as a key effector of Semaphoring 3E-PlexinD1 signaling. J Cell Biol, 205(4):573-590. (Download PDF)
Podcast-biobytes May 26, 2014
 

Straub, C., Tritsch, NX, Hagan NA, Gu, C., Sabatini, B., (2014) Multiphasic modulation of cholinergic interneurons by nigrostriatal afferents. J Neuroscience, 34(25):8557-6 (Download PDF)
 

Tritsch, N., Oh, W., Gu, C., Sabatini, B., (2014) Midbrain dopamine neurons sustain inhibitory transmission using plasma membrane uptake of GABA, not synthesis. elife. 3:e01936. doi: 10.7554/eLife.01936. (Download PDF)
 

Oh, W., Gu, C., (2013) Establishment of neurovascular congruency in the mouse whisker system by an independent patterning mechanism. Neuron. 80(2):458-469. (Download PDF)
Supplementary Marterials

Preview in Neuron 
Also see news report on the study.
 

Gu, C., Giraudo E., (2013) The role of semaphorins and their receptors in vascular development and cancer.  Exp Cell Res. 319(9):1306-16. (Download PDF)
 

Oh, W., Gu, C., (2012) The role and mechanism-of-action of Sema3E and Plexin-D1 in vascular and neural development. Seminars in Cell and Developmental Biology. 24(3):156-62. (Download PDF)
 

Kwon, H., Kozorovitskiy, Y., Oh, W., Peixoto, R., Akhtar, N., Saulnier, J. L., Gu, C., Sabatini, B.L., (2012) Cortical synaptogenesis and excitatory synapse number are determined via a Neuroligin-1-dependent intercellular competition. Nature Neuroscience. 15(12):1667-74. (Download PDF)
Supplementary Materials 


Ding*, J., Oh*, W., Sabatini, B.L., Gu, C., (2011) Semaphorin3E-Plexin-D1 signaling controls pathway-specific synapse formation in the striatum. Nature Neuroscience. 15(2):215-23. (Download PDF)
Supplementary Materials
 

Kim*, J., Oh*, W., Gaiano, N., Yoshida, Y., Gu, C., (2011) Semaphorin 3E-Plexin-D1 signaling regulates VEGF function in developmental angiogenesis via a feedback mechanism.Genes & Development. 25 (13):1399-411. (Download PDF)

Selected to be the cover.
Also see news report on the study.
 

De Almodovar,C.,Fabre, P., Knevels, E., Coulon,C., Segura, I., Haddick, P., Aerts, L., Delattin, N., Strasser, G., Oh, W., Lange, C., Vinckier, S.,  Haigh, J., Fouquet,C., Henderson, C.,  Gu, C., Alitalo, K., Castellani, V., Tessier-Lavigne, M., Chedotal, A Charron, F., Carmeliet, P., (2011) VEGF mediates commissural axon chemoattraction through its receptor Flk1. Neuron. 70(5):966-78. (Download PDF)
 

Piper, M., Plachez, C., Zalucki,O., Fothergill, T., Goudreau, G., Erzurumlu, R., Gu, C., Richards LJ., (2009) Neuropilin 1-Sema Signaling Regulates Crossing of Cingulate Pioneering Axons during Development of the Corpus Callosum. Cereb Cortex. Suppl 1:i11-21. (Download PDF)
 

Gelfand, M. V., Hong, S., Gu, C., (2009) Guidance from above: common cues direct distinct signaling outcomes in vascular and neural patterning. Trends in Cell Biology. 19(3):99-110. (Download PDF)
 

Huber, A. B., Kania, A., Tran, T.S., Gu, C., De Marco, N., Lieberam, I., Johnson, D.,Jessell, T.M., Ginty, D.D., Kolodkin, A.L., (2005) Distinct roles for secreted semaphorin signaling in spinal motor axon guidance. Neuron. 48(6): 949-64. (Download PDF)
 

Gammill, L.S., Gonzalez, C., Gu, C., Bronner-Fraser, M., (2005) Guidance of trunk neural crest migration requires Neuropilin-2/Semaphorin3F signaling. Development. 133(1):99-106. (Download PDF)
 

Mukouyama, Y., Gerber, H., Ferrara, N., Gu, C., Anderson, D.J., (2005) Peripheral nerve-derived VEGF  promotes  arteriogenesis via Neuropilin-1-mediated positive-feedback. Development. 132(5):941-952. (Download PDF)
 

Gu, C., Yoshida, Y., Livet, J.,Reimert, D.V., Mann, F., Merte, J., Henderson, C.E., Jessell, T.M., Kolodkin, A.L., Ginty, D.D. (2005) Semaphorin3E and Plexin-D1 control vascular pattern independently of Neuropilins. Science. 307 (5707):265-268. (Download PDF)
Supplementary Materials
 

Schwarz, Q., Gu, C., Fujisawa , H., Sabelko, K., Golding, M., Cheng, H., Marina Gertsenstein, M., Nagy, A., Taniguchi, M., Kolodkin, A.L., Ginty, D.D., Shima, D.T., Ruhrberg, C. (2004) Vascular endothelial growth factor cooperates with Sema3A to pattern different compartments of the facial nerve. Genes & Development. 18 (22): 2822-2834. (Download PDF)
Supplementary Materials: Fig. 1, Fig. 2, Legends.
 

Gu, C., Rodriguez, E.R., Reimert, D.V., Shu, T., Fritzsch, B., Richards, L.J., Kolodkin, A.L., Ginty, D.D. (2003) Neuropilin-1 conveys Semaphorin and VEGF signaling during neural and cardiovascular development.Developmental Cell. 5 (1):45-57. (Download PDF)
 

Gu, C., Limberg, B.J., Whitaker, G.B., Perman, B., Leahy, D.J., Rosenbaum, J.S., Ginty, D.D., Kolodkin, A.L. (2002) Characterization of neuropilin-1 structural features that confer binding to semaphorin 3A and vascular endothelial growth factor 165. J Biol Chem. 17; 277(20):18069-76. (Download PDF)
 

Gu, C., Ma, Y., Benjamin, J., Littman, D., Chao, M.V., and Huang, X. (2000) Apoptotic signaling through the -adrenergic receptor: A new Gs coupled mechanism. J. Biol Chem. 275(27):20726-33.
 

Gu, C., Casaccia-Bonnefil, P., Srinivasan, A., and Chao, M.V.(1999) Oligodendrocyte apoptosis mediated by caspase activation. J. Neurosci. 19(8):3043-3049.
 

Casaccia-Bonnefil, P., Gu, C., Khursigara, G., and Chao, M.V. (1999) The p75 neurotrophin receptor as a modulator of survival and death decisions. Microsc Res Tech. 45(4-5):217-24.
 

Casaccia-Bonnefil, P., Gu, C., and Chao, M.V. (1999) Neurotrophins in cell survival/death decisions. Adv Exp Med Biol. 468:275-82.
 

Gu, C., Castellino, A., Chan, J., and Chao, M.V. (1998) BRE: A modulator of TNF-alpha action. FASEB J. 12:1101-1108.